Biotechnological Application of Plant Tissue Culture to Forestry in India

Forest tree tissue culture in India is being carried out in several universities and research institutions. The main thrust of these activities is directed towards micropropagation, using explants from both adult and juvenile embryonic or seedling tissues. The tree species used in these studies cover a wide spectrum which include desert and arid zone trees, leguminous trees and also trees used for timber, fuel, paper and pulp and in rayon industries.
The micropropagation of hardwood forest species is still a controversial issue because of the high cost of plantlet production, which is mainly a result of the labor-intensive stages in the process, and because of the uncertainties regarding the likely gains. This requires careful field evaluation of tissue culture-raised plants with respect to growth rates and wood quality, depending on the end-uses.
The results of small-scale outplantings at the National Chemical Laboratory with tissue culture-raised plants of Dendrocalanuts strictus, Eucalyptus spp., Salvadora persica, and Tamarindus indica have been presented, together with results of the different approaches followed in making the micropropagation processes cost-effective and beneficial for use in forestry programs.
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References
- Aitken-Christie, J. and Jones, C., 1987, Towards automation: Radiata pine shoots hedges in vitro, Plant Cell Tissue Organ Cult., 8: 185–196. ArticleGoogle Scholar
- Anand, M. and Bir, S.S., 1984, Organogenetic differentiation in tissue cultures of Dalbergia larifolia, Cuir. Sci., 53: 1305–1307. Google Scholar
- Ancja, S. and Atal, C.K., 1969, Plantlet formation from tissue culture from lignotuber of Eucalyptus citriodora, Cuir. Sci., 38: 69. Google Scholar
- Bapat, V.A. and Rao, P.S., 1979, Somatic embryogenesis and plantlet formation in tissue cultures of sandalwood (Santalunr album), Ann. Bot. (Load.), 44: 629–630. Google Scholar
- Bapat, V.A., Gill, R. and Rao, P.S., 1985, Regeneration of somatic embryos and plantlet from stem callus protoplast of sandalwood tree (Santaluor album), Crm. Sci., 54: 978–982. Google Scholar
- Bhatnagar, S.P., Singh, M.N. and Kapur, N., 1983, Preliminary investigations on organ differentiation in tissue cultures of Cedrus deodara and Pines ro.vburghii, Indian J. Exp. Biol., 21: 524–526. Google Scholar
- Bonga, J.M. and Durzan, J., 1987 (eds.), Cell and Tissue Culture in Forestry, Vols. 1, 2 and3, Martinus Nijhoff, Dordrecht. Google Scholar
- Brando, L.G., 1984, The new Eucalyptus forest, The Morus Wallenberg Foundation Symposia Proceedings, Falun, Sweden, pp. 3–15. Google Scholar
- Brown, C.L., 1981, Application of tissue culture technology to production of woody biomass. In: PPOC. Planning Gp. B. pp. 1–18, Int. Energy Agency, Brighton. Google Scholar
- Chadha, Y.R. (ed.). In: Wealth of India, Vol. 9, pp. 193–195, Publications and Information Directorate, CSIR, New Delhi. Google Scholar
- Chaturvedi, A.N. and Venkatraman, K.G., 1973, Volume and weight table for Lucalvptus hybrid, Indian Forester; 99: 599–608. Google Scholar
- Cheema, G.S., 1987, Tissue culture of poplars with particular reference to improvement of Poplars in India. In: Proc. Svmp. Genetic Manipulation of Woody Plants, pp. 461, Michigan State Univ., Michigan. Google Scholar
- Corley, R.H.V., Lee, C.H., Law, L.H. and Wong, C.Y., 1986, Abnormal flower development in oil palm clones, Planter (Kuala Lumpur), 62: 233–240. Google Scholar
- Datta, K. and Datta, S.K., 1985, Auxin + KNO3 induced regeneration of leguminous tree Leucoma leucoccpinala through tissue culture, CWT. Sci., 54: 248–250. Google Scholar
- Datta, S.K., Datta, K. and Pramanik, T., 1983, In vitro clonal multiplication of mature trees of Dalbergia sissoo, Plant Cell Tissue Organ Cult., 2: 15–20. ArticleGoogle Scholar
- Dhawan, V. and Bhojwani, S.S., 1985, In vino vegetative propagation of Leucaena leucocephala, Plant Cell Rep., 4: 315–318. ArticleGoogle Scholar
- Dhir, K.K., Angrish, R. and Bajaj M., 1984, Micropropagation of Saliv babvlonica through in vino shoot proliferation, Proc. Indian Acad. Sci. (Plant Sci.), 93: 655–660. ArticleGoogle Scholar
- Frampton, L.J. and Lsik, K., 1986, Comparison of field growth among loblolly pine seedlings and three plant types produced in vitro. In: Research and Development Conference, TAPPI Press, Raleigh. Google Scholar
- Franclet, A. and Boulay, M., 1983, Micropropagation of frost-resistant Eucaltpms clones, Aust. For. Res., 13: 83–89. Google Scholar
- Gharyal, P.K. and Maheshwari, S.C., 1981, In vitro differentiation of somatic embryoids in a leguminous treeAlbia_ia Iebbeck, Naturwissenschaften, 68: 379–380. ArticleGoogle Scholar
- Gharyal, P.K., Rashid, A. and Maheshwari, S.C., 1983, Production of haploid plants in anther cultures of Albiz_ia Iebbeck, Plant Cell Rep., 2: 308–309. ArticleGoogle Scholar
- Goyal, Y. and Arya, H.C., 1981, Differentiation in cultures of Prosopiscmeraria, CWT. Sci., 50: 468–469. Google Scholar
- Goyal, Y. and Arya, H.C., 1984, Tissue culture of desert trees: I Clonal multiplication of Prosopis cineraria by bud culture, J. Plant Pirvsiol., 115: 183–189. ArticleGoogle Scholar
- Grewal, S., Ahuja, A. and Atal C.K., 1980, M vino proliferation of shoot apices of Eucaltptuscitriodora, Indian J. Exp. Biol., 18: 775–776. Google Scholar
- Gupta, P.K. and Mascarenhas, A.F., 1987, Eucalyptus. In: Cell and Tissue Culture in Foresny Vol. 3 ( J.M. Bonga and D.J. Durzan, eds.), pp. 385–399, Martinus Nijhoff, Dordrecht. Google Scholar
- Gupta, P.K., Mascarenhas, A.F. and Jagannathan, V., 1981, Tissue culture of forest trees–Clonal propagation of mature trees of Eucalyptus citriodora Hook by tissue culture, Plant Sci. Leu., 20: 195–201. ArticleGoogle Scholar
- Gupta, P.K., Mehta, U.J. and Mascarenhas, A.F., 1983, A tissue culture method for rapid multiplication of mature trees of Eucalplus torelliana and E. camaldulensis, Plant Cell Rep., 2: 296–299. ArticleGoogle Scholar
- Gupta, P.K., Nadgir A.L., Mascarenhas A.F. and Jagannathan V. 1980, Tissue culture of forest trees–Clonal multiplication of Tectona grandis (Teak) by tissue culture, Plant Sci. Leu., 17: 259–268. ArticleGoogle Scholar
- Gupta, P.K., Nadgauda, R.S., Hendre, R.R., Mascarenhas, A.F. and Jagannathan, V., 1978. In: Proc. All India Svmp. and 3rd Canf. of Plant Tissue Cult., pp. 63–64, M.S. Univ., Baroda. Google Scholar
- Hasnain, S. and Cheliak, W., 1986a, Tissue culture in forestry: economic and genetic potential, The Foresm’ Chronicle (August issue), 219–225. Google Scholar
- Hasnain, S., Pigeon, R. and Overend, R.P., 1986b, Economic analysis of the use of tissue culture for rapid forest improvement, The Forestry Chronicle (August issue), 240–245. Google Scholar
- Jackson, M.L., 1967, Soil Chemical Analysis, Prentice - Hall of India, New Delhi. Google Scholar
- Jaiswal, V.S. and Narayan, P., 1985, Regeneration of plantlets from the callus of stem segments of adult plant of Ficus religiosa, Plant Cell Rep., 4: 256–258. ArticleGoogle Scholar
- Khattar, S. and Mohan Ram, H.Y., 1983, Organogenesis and plantlet formation in vitro in Sesbania grandiflora, Indian. J. Eli). Biol., 21: 252–253. Google Scholar
- Khuspe, S.S., Gupta, P.K., Kulkarni, D.K., Mehta, U.J. and Mascarenhas, A.F., 1987, Increased biomass production by tissue culture of Eucalyptus., Can. J. For., 17. Google Scholar
- Konar, R.N., 1975, In vitro studies on Pinus I1. The growth and morphogenesis of cell cultures from Pinus gerardiana, Phvtonrorphologt, 15: 137–140. Google Scholar
- Konar, R.N. and Oberoi, Y.P., 1965, In vino development of embryoids on cotyledons of Biota orientalis, Phvtomorphology, 15: 137–140. Google Scholar
- Konar, R.N. and Singh, M.N., 1980, Induction of shoot buds from tissue cultures of Mints wallichiana, Z. Pflan_enphvsiol., 99: 173–177. Google Scholar
- Kotwal, M., Gupta, P.K. and Mascarenhas, A.F., 1983, Rapid multiplication of Sapiurn sebiferunt by tissue culture, Plant Cell Tissue Organ Cult., 2: 133–139. ArticleGoogle Scholar
- Kulkarni, D.K., Gupta, P.K. and Mascarenhas, A.F., 1984, Tissue culture studies on Leucaena leucocephala, Leucaena Res. Rep., 5: 37–39. Google Scholar
- Kulkarni, V.M., Gupta, P.K., Mehta, U.J. and Mascarenhas, A.F., 1981, Tissue culture of woody trees: clonal propagation of Tamarindus indica Linn. (Tamarind) by tissue culture, Abst. of the 6th All India Plant Tissue Culture Conference, Dept. of Bot., Pune University. Google Scholar
- Lakshmi Sita, G., 1979, Morphogenesis and plant regeneration from cotyledonary cultures of Eucalyptus, Plant Sci. Lett., 14: 63–68. ArticleGoogle Scholar
- Lakshmi Sita, G. and Shobha Rani, B., 1985, In vitro propagation in Eucalyptus grandis by tissue culture, Plant Cell Rep.,4: 78–80. ArticleGoogle Scholar
- Lakshmi Sita, G., Raghav Ram, N.V. and Vaidyanathan C.S., 1979, Differentiation of embryoids and plantlets from shoot callus of sandalwood, Plant Sci. Lett., 15: 265–270. ArticleGoogle Scholar
- Lakshmi Sita, G., Raghav Ram, N.V. and Vaidyanathan, C.S., 1980, Triploid plants from en- dosperm cultures of sandalwood by experimental embryogenesis, Plant Sci. Leu., 20: 63–69. ArticleGoogle Scholar
- Mascarenhas, A.F., Hazra, S., Potdar, U., Kulkarni, D.K. and Gupta, P.K., 1982, Rapid clonal multiplication of mature forest trees through tissue culture. In: Plant Tissue Culture. ( A. Fujiwara, ed.), pp. 719–720. Jpn. Assoc. Plant Tissue Cult., Tokyo. Google Scholar
- Mascarenhas, A.F., Kendurkar, S.V., Gupta, P.K., Khuspe, S.S. and Agrawal, D.C., 1987, Teak. In: Cell and Tissue Culture in Forestry, Vol. 3, (J.M. Bonga and D.J. Durzan, eds.), pp. 300–315, Martinus Nijhoff, Dordrecht. Google Scholar
- Mascarenhas, A.F., Nair, S., Kulkarni, V.M., Agrawal, D.C., Khuspc, S.S. and Mehta, U.J., 1987, Tamarind, In: Cell and Tissue Culture in Forestry, Vol. 3 ( J.M. Bonga and D.J. Durzan, eds.), pp. 316–330, Martinus Nihoff, Dordrecht. Google Scholar
- Mascarenhas, A.F., Khuspe, S.S., Nadguada R.S., Gupta, P.K. and Khan, B.M., 1988, Potential of cell culture in plantation forestry programmes. In: Genetic Manipulation of Woody Plants, pp. 391–412, Plenum Press, New York. ChapterGoogle Scholar
- Mathur, I. and Chandra, N., 1983, Induced regeneration in stem explants of Acacia nilotica, Con•. Sci., 52: 882–883. Google Scholar
- Mehra, P.N. and Cheema G.S., 1980, Clonal multiplication in vitro of Himalayan Poplar (Populos ciliata), Phvtomorphology, 30: 336–343. Google Scholar
- Mehra, P.N. and Verena, V., 1981, Callus induction and shoot bud formation on cotyledons of West Himalayan spruce (Picea smithiana), Phvtomorpholog, 31: 60–69. Google Scholar
- Mehta, U., Ramanuja Rao, I.V. and Mohan Ram, H.Y., 1982, Somatic embryogenesis in bamboo. In: Plant Tissue Culture ( A. Fujiwara, ed.), pp. 109–110, Jpn. Assoc. Plant Tissue Cult., Tokyo. Google Scholar
- Mahatre, M., Bapat, V.A. and Rao, P.S., 1985, Regeneration of plants from the culture of leaves and axillary buds in mulberry (Morus indica L.), Plant Cell Rep., 4: 78–80. ArticleGoogle Scholar
- Mukhopadhyay, A. and Mohan Ram, H.Y., 1981, Regeneration of plantlets from excised roots of Dalbergia sissoo, Indian J. Exp. Biol., 19: 1113–1115. Google Scholar
- Muralidharan, E.M. and Mascarenhas, A.F., 1987a, In vitro plantlet formation by organogenesis in Eucalyptus camaldulensis and by somatic embryogenesis in E. cinriodora, Plant Cell Rep., 6: 256–260. ArticleGoogle Scholar
- Muralidharan, E.M. and Mascarenhas, A.F., 1987b, In vitro morphogenesis in Azadiracloa indica and Eucalyptus citriodora. In: Abst. Intl. Workshop on Tissue Culture and Biotechnology of Medicinal and Aromatic Plants,CIMAP, Lucknow (in press). Google Scholar
- Nadgir, A.L., Phadke, C.H., Gupta, P.K., Parasharami, V.A., Nair, S. and Mascarenhas, A.F., 1984, Rapid multiplication of bamboo by tissue culture, Sil, ae Geneti. 33: 221–223. Google Scholar
- Narayan, P. and Jaiswal, V.S., 1985, Plantlet regeneration from leaflet callus of/la/dime/1w indica, J. The Sci., 4: 65–68. Google Scholar
- Nataraja, K. and Sudhadevi, A.M., 1985, Induction of plantlet from seedling explants of Dalbergia latifolia h vitro, Bitr. Biot Pflana., 59: 341–350. Google Scholar
- Olson, S.R., Cole, G.V., Watanabe, F.W. and Dean, L.A., 1954, Estimation of available phosphorus in soils by extraction with sodium bicarbonate, USDA, Cire., pp. 939. Google Scholar
- Perur, N.G., Subramaniam, C.K., Muhr, G.R. and Ray, H.E., 1973, Soil fertility evaluation to serve Lydian fanners, Univ. Agric. Sci., Bangalore, pp. 124. Google Scholar
- Rao, LU., Rao, I.V.R. and Narang, V., 1985, Somatic embryogenesis and regeneration of plants in the bamboo Dendrocalantus strictus, Plant Cell Rep., 4: 191–194. ArticleGoogle Scholar
- Rao, K.S., 1986, Plantlets from somatic callus tissue of East Indian Rosewood, (Dalbergia tatifolia), Plant Cell Rep., 5: 199–202. ArticleGoogle Scholar
- Rao, K.S., and Venkateswara, R, 1985, Tissue culture of forest trees: Clonal multiplication of Eucalyptus grandis, Plant Sci., 40: 51–55. ArticleGoogle Scholar
- Rao, P.S. and Bapat, V.A., 1978, Vegetative propagation of sandalwood plants through tissue culture, Can. J. Bot., 56: 1153–1156. ArticleGoogle Scholar
- Rao, P.S. and Ozias-Akins, P., 1985, Plant regeneration through somatic embryogenesis in protoplast cultures of sandalwood (Santalum album), Protoplasma, 124: 80–86. ArticleGoogle Scholar
- Rao, S.M., 1987, In vitro studies on Subvadora persica Linn. M.Sc. Thesis, Univ. Pune, India. Google Scholar
- Redenbaugh, K., Paasch, B.D., Nichol, J.W., Kossler, M.E., Vess, P.R. and Walker, K.A., 1986, A gel encapsulation system for alfalfa somatic embryos, Biotechnology, 4: 797–801. ArticleGoogle Scholar
- Rydholm, S.A., 1965. Pulping Processes, John Wiley and Sons, New York. Google Scholar
- Smith, D.M., 1955, A comparison of two methods for determining the specific gravity of small samples of secondary growth of Douglas-fir, U.S.F.P.L. Ref. No. 2033, pp. 13–20. Google Scholar
- Srivastava, P.S., 1973, Formation of triploid plantlets in endosperm culture of Putranjiva roxburghii, Z. Pjlanenphysiol., 69: 270–273. Google Scholar
- Upadhyay, S. and Chandra, N., 1983, Shoot and plantlet formation in organ and callus culture of Albin is lebbeck, Ann. Bot. (Loud.), 52: 421–424. Google Scholar
- Walkely, A. and Black, T.A., 1934, Determination of organic carbon by rapid titration method, Soil Sci., 37: 29–35. ArticleGoogle Scholar
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Authors and Affiliations
- Biochemical Sciences Division, National Chemical Laboratory, Pune, 411 008, India A. F. Mascarenhas, S. S. Khuspe, R. S. Nadgauda, P. K. Gupta, E. M. Muralidharan & B. M. Khan
- A. F. Mascarenhas